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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?，这一点在爱思助手下载最新版本中也有详细论述
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